SLC25A15 Polyklonaler Antikörper

SLC25A15 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15317-1-AP

Synonyme

D13S327, HHH, ORC1, ORNT1, SLC25A15

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15317-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15317-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 15317-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 15317-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody (15317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe, Maus-Magengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15317-1-AP bindet in WB, IHC, IF, ELISA SLC25A15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A15 fusion protein Ag7563
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC002702
• Gene symbol: SLC25A15
• Gene ID (NCBI): 10166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A15 gene encodes a 301-amino-acid-long mitochondrial carrier protein termed ORC1., which catalyzes the transport of cytosolic ornithine into mitochondria in exchange for citrulline (PMID: 19242930). Some mutations in the SLC25A15 gene have been reported to break the ornithine cycle and be related with human hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome (PMID: 19242930, 24721342). This antibody detected SLC25A15 at an apprarant molecular mass about 33 kDa.