SLC25A17 Monoklonaler Antikörper

SLC25A17 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D1D2

Kat-Nr. : 67635-1-Ig

Synonyme

PMP34, SLC25A17

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Galerie der Validierungsdaten

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  WB analysis using 67635-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67635-1-Ig (SLC25A17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of U2OS using 67635-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67635-1-Ig (SLC25A17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67635-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67635-1-Ig (SLC25A17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67635-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67635-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67635-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67635-1-Ig bindet in WB, IHC, ELISA SLC25A17 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLC25A17 fusion protein Ag24456
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; peroxisomal membrane protein, 34kDa), member 17
• Berechnetes Molekulargewicht: 307 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC012998
• Gene symbol: SLC25A17
• Gene ID (NCBI): 10478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A17 (solute carrier family 25 member 17), is a kind of peroxisomal transporter for multiple cofactors such as CoA, FAD, FMN and AMP. It May catalyze the transport of free CoA, FAD and NAD+ from the cytosol into the peroxisomal matrix by a counter-exchange mechanism. SLC25A17 is the only member of the mitochondrial carrier family localized to peroxisomal. (PMID: 22185573, 32266253)