SLC25A20 Polyklonaler Antikörper

SLC25A20 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 19363-1-AP

Synonyme

CACT, CAC

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• 

  WB analysis of mouse heart using 19363-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19363-1-AP (SLC25A20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19363-1-AP (SLC25A20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19363-1-AP (SLC25A20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19363-1-AP (SLC25A20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19363-1-AP

  Immunofluorescent analysis of Hela cells, using SLC25A20 antibody 19363-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 19363-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SLC25A20 antibody 19363-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Saos-2 cells, humanes Hodengewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below

Produktinformation

19363-1-AP bindet in WB, IHC, IF/ICC, ELISA SLC25A20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A20 fusion protein Ag6896
• Vollständiger Name: solute carrier family 25 (carnitine/acylcarnitine translocase), member 20
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC001689
• Gene symbol: SLC25A20
• Gene ID (NCBI): 788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.; Authors - Jin-Ho Koh
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
mouse	WB	Nutrients; The Enhancement of Acylcarnitine Metabolism by 5-Heptadecylresorcinol in Brown Adipose Tissue Contributes to Improving Glucose and Lipid Levels in Aging Male Mice; Authors - Kuiliang Zhang
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu
mouse	WB	Front Physiol; PPARδ Attenuates Alcohol-Mediated Insulin Resistance by Enhancing Fatty Acid-Induced Mitochondrial Uncoupling and Antioxidant Defense in Skeletal Muscle.; Authors - Jin-Ho Koh
mouse	WB	Cell Rep; Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.; Authors - Nicole M Sayles