SLC25A22 Polyklonaler Antikörper

SLC25A22 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25402-1-PBS

Synonyme

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  WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 25402-1-AP (same clone as 25402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 25402-1-AP (same clone as 25402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 25402-1-AP (same clone as 25402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 25402-1-AP (same clone as 25402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLC25A22 antibody (25402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25402-1-AP (SLC25A22 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

• 

  IF Staining of COS-7 using 25402-1-AP (same clone as 25402-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed COS-7 cells using 25402-1-AP (SLC25A22 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25402-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA SLC25A22 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A22 fusion protein Ag19958
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier: glutamate), member 22
• Berechnetes Molekulargewicht: 323 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC023545
• Gene symbol: SLC25A22
• Gene ID (NCBI): 79751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)