SLC25A22 Rekombinanter Antikörper

SLC25A22 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250788B11

Kat-Nr. : 86163-1-RR

Synonyme

GC 1, GC1, GC-1, Glutamate/H(+) symporter 1, NET44

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  WB analysis using 86163-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86163-1-RR (SLC25A22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86163-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86163-1-RR against Human SLC25A22 were performed. The affinity constant is 1.13 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86163-1-RR bindet in WB, ELISA SLC25A22 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC25A22 fusion protein Ag19958
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier: glutamate), member 22
• Berechnetes Molekulargewicht: 323 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC023545
• Gene symbol: SLC25A22
• Gene ID (NCBI): 79751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)