SLC25A24 Polyklonaler Antikörper

SLC25A24 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14669-1-AP

Synonyme

APC1, MCSC1, Mitochondrial adenyl nucleotide antiporter SLC25A24, Mitochondrial ATP-Mg/Pi carrier protein 1, Mitochondrial Ca(2+)-dependent solute carrier protein 1

---

• 

  WB analysis of HEK-293 using 14669-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14669-1-AP (SLC25A24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14669-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14669-1-AP (SLC25A24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14669-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14669-1-AP (SLC25A24 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 14669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14669-1-AP (SLC25A24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14669-1-AP (SLC25A24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 14669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 14669-1-AP (SLC25A24 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Plazenta-Gewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14669-1-AP bindet in WB, IHC, IF/ICC, ELISA SLC25A24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A24 fusion protein Ag6334
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC068561
• Gene symbol: SLC25A24
• Gene ID (NCBI): 29957
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A24 (solute carrier family 25 member 24) is a gene encoding a mitochondrial ATP-Mg/phosphate transporter. It belongs to the mitochondrial carrier protein family and is mainly involved in mitochondrial energy metabolism and calcium signal regulation.

Publikationen

Species	Application	Title
rat	WB	Sci Rep; Microsecond pulsed electric fields induce myocardial ablation by secondary mitochondrial damage and cell death mechanisms; Authors - Qiqi Gao