ANT1/2 Rekombinanter Antikörper

ANT1/2 Rekombinant Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230247B1

Kat-Nr. : CL750-83104

Synonyme

SLC25A5, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2

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  WB analysis using CL750-83104

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83104 (SLC25A5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of PC-3 using CL750-83104

  1x10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody (CL750-83104, Clone:230247B1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, Mauslebergewebe, Neuro-2a-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in FC (Intra): PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-83104 bindet in WB ANT1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ANT1/2 fusion protein Ag11956
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Berechnetes Molekulargewicht: 298 aa, 33 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC056160
• Gene symbol: SLC25A5
• Gene ID (NCBI): 292
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)