SLC25A6-Specific Polyklonaler Antikörper

SLC25A6-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 51031-1-AP

Synonyme

SLC25A6, AAC3, ADP,ATP carrier protein 3, ADP/ATP translocase 3, ADP/ATP translocase 3, N-terminally processed

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  WB analysis using 51031-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51031-1-AP (SLC25A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 51031-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 51031-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 51031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 51031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, K-562-Zellen, NCI-H1299-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

51031-1-AP bindet in WB, IHC, ELISA SLC25A6-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC031912
• Gene symbol: SLC25A6
• Gene ID (NCBI): 293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).

Publikationen

Species	Application	Title
		Int J Oncol; Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.; Authors - Mohamed E M Saeed
human	WB	MethodsX; Complete solubilization of mammalian cells in lysates; Authors - Izabela Bednarska