SLC26A2 Polyklonaler Antikörper

SLC26A2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 27759-1-AP

Synonyme

DTD, DTDST

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  WB analysis of mouse colon using 27759-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 27759-1-AP (SLC26A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 27759-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 27759-1-AP (SLC26A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27759-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27759-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27759-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 27759-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 27759-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLC26A2 antibody (27759-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of U2OS using 27759-1-AP

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human SLC26A2 (27759-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 3 publications below

Produktinformation

27759-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA SLC26A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC26A2 fusion protein Ag26521
• Vollständiger Name: solute carrier family 26 (sulfate transporter), member 2
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC059390
• Gene symbol: SLC26A2
• Gene ID (NCBI): 1836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,human,canine	IF	Biomater Res; ι-Carrageenan nanocomposites for enhanced stability and oral bioavailability of curcumin.; Authors - Joo Young Lee
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
human	WB	iScience; Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients; Authors - Xiao-Hu Zhao
human	IF	Cell Stem Cell; Bioengineered human colon organoids with in vivo-like cellular complexity and function; Authors - Olga Mitrofanova
human	WB	PLoS One; Screening and validation of 3'-Methoxydaidzein as a therapeutic agent in ulcerative colitis based on disulfidptosis-associated molecular clusters; Authors - Jie Yuan