SLC27A3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC27A3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12943-1-AP

Synonyme

ACSVL3, FATP 3, FATP3, Fatty acid transport protein 3, SLC27A3, UNQ367/PRO703, VLCS 3

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Galerie der Validierungsdaten

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  WB analysis using 12943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12943-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 12943-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12943-1-AP

  IP Result of anti-SLC27A3 (IP:12943-1-AP, 4ug; Detection:12943-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maushodengewebe, MCF-7-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Mammakarzinomgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

12943-1-AP bindet in WB, IP, IHC, ELISA SLC27A3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC27A3 fusion protein Ag4015
• Vollständiger Name: solute carrier family 27 (fatty acid transporter), member 3
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 70-79 kDa
• GenBank-Zugangsnummer: BC029792
• Gene symbol: SLC27A3
• Gene ID (NCBI): 11000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC27A3, also named as FATP3, is a member of the solute carrier 27A (SLC27A) gene family which encodes fatty acid transport proteins. SLC27A3 is expressed in human neural stem cells and plays a key role in the developmental stage of the central nervous system. It is associated with autism spectrum disorders (ASD) that caused by impaired early brain development.(PMID: 26548558).

Publikationen

Species	Application	Title
human	WB	Nat Med; PMID: 26950361; Authors - Cholsoon Jang ; KD Validated
mouse	WB	Cancers (Basel); In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer.; Authors - Megan C Madonna
mouse	WB	Eur J Pharmacol; A novel therapy for hepatic cholestasis treatment-the combination of rosiglitazone and fenofibrate; Authors - Yuanli Chen
human,mouse	WB,IHC	Front Immunol; Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease.; Authors - Yuepeng Zhang
pig	WB	Mol Reprod Dev; Cloned pig fetuses exhibit fatty acid deficiency from impaired placental transport.; Authors - Zheng Ao
mouse	WB	Int J Mol Sci; Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development; Authors - Mingfa Ling