• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SLC30A1 Polyklonaler Antikörper

SLC30A1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 22661-1-AP

Synonyme

Zinc transporter 1, ZNT1, ZnT-1

Filter:
  • Western Blot (WB) analysis of various lysates using SLC30A1 Polyclonal antibody (22661-1-AP)

    WB analysis using 22661-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22661-1-AP (SLC30A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HeLa membrane protein, HepG2 total crude protein, HepG2 membrane protein

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 1 publications below

Produktinformation

22661-1-AP bindet in WB, IHC, ELISA SLC30A1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SLC30A1 fusion protein Ag18567
Vollständiger Name solute carrier family 30 (zinc transporter), member 1
Berechnetes Molekulargewicht 507 aa, 55 kDa
Beobachtetes Molekulargewicht63~75 kDa
GenBank-ZugangsnummerBC121015
Gene symbol SLC30A1
Gene ID (NCBI) 7779
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). ZnT1 is located in the plasma membrane and is responsible for Zn export from the cytosol to the extracellular space during periods of elevated cytosolic Zn. Western analysis detected a higher band at∼63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319, 15753530).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SLC30A1 antibody 22661-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Eur J Histochem

HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum.

Authors - Li Niu
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

James (Verified Customer) (10-01-2025)

Excellent antibody. Have used at 1:2000 for Western blotting, detection by chemiluminescence.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human In vitro Cancer Cells
FH

David (Verified Customer) (09-15-2025)

Concentration used 1:5000. works pretty good. Strong clean signal.

  • Applications: Western Blot