ZnT3 Polyklonaler Antikörper

ZnT3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17363-1-AP

Synonyme

SLC30A3, Zinc transporter 3, ZnT 3, ZNT3

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Galerie der Validierungsdaten

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  WB analysis using 17363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17363-1-AP (ZnT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17363-1-AP (ZnT3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17363-1-AP (ZnT3 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

17363-1-AP bindet in WB, IHC, IF, ELISA ZnT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZnT3 fusion protein Ag10735
• Vollständiger Name: solute carrier family 30 (zinc transporter), member 3
• Berechnetes Molekulargewicht: 388 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 48 kDa
• GenBank-Zugangsnummer: BC028358
• Gene symbol: SLC30A3
• Gene ID (NCBI): 7781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZnT3, encoded by SLC30A3, is a member of SLC30A family which is zinc transporter sequestrating cytosolic zinc into intracellular compartments or effluxing zinc to the extracellular space. Excessive zinc level can cause neuron apoptosis and lead to cognitive impairments. Znt3 facilitates the transport and storage of zinc in synaptic vesicles. Abnormal ZnT3 expression has been linked to some neurodegenerative disorders.

Publikationen

Species	Application	Title
mouse	WB	Acta Neuropathol Commun; Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.; Authors - Alexandra Grubman
	WB	J Cereb Blood Flow Metab; MicroRNA-30a regulates acute cerebral ischemia-induced blood-brain barrier damage through ZnT4/zinc pathway.; Authors - Peng Wang
mouse	IHC	Cell Biosci; Disruption of zinc transporter ZnT3 transcriptional activity and synaptic vesicular zinc in the brain of Huntington's disease transgenic mouse.; Authors - Li Niu
human	WB	Int J Mol Sci; MicroRNA-5572 Is a Novel MicroRNA-Regulating SLC30A3 in Sporadic Amyotrophic Lateral Sclerosis.; Authors - Hisaka Kurita
human, mouse	WB, IHC, IF	J Neurosci Res; Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis.; Authors - Masayuki Kaneko
human	WB,IF	Biochem Biophys Res Commun; Protective roles of SLC30A3 against endoplasmic reticulum stress via ERK1/2 activation.; Authors - Hisaka Kurita