ZnT4 Rekombinanter Antikörper

ZnT4 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250909D12

Kat-Nr. : 86336-1-PBS

Synonyme

SLC30A4, Probable proton-coupled zinc antiporter SLC30A4, ZnT 4, ZnT-4

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  WB analysis using 86336-1-RR (same clone as 86336-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86336-1-RR (ZnT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86336-1-PBS in a different storage buffer formulation.

• 

  Sample test of MP02364-1

  The mean ZnT4 concentration was determined to be 45.6 ng/mL in LNCaP cell extract based on a 1.2 mg/mL extract load.

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  IHC staining of human prostate cancer using 86336-1-RR (same clone as 86336-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86336-1-RR (ZnT4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86336-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02364-1

  Cytometric bead array standard curve of MP02364-1, ZnT4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86336-2-PBS. Detection antibody: 86336-1-PBS. Standard: Ag3313. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86336-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86336-1-RR against Human ZnT4 were performed. The affinity constant is 3.87 nM.

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Geprüfte Anwendungen

Produktinformation

86336-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA, Sample test ZnT4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZnT4 fusion protein Ag3313
• Vollständiger Name: solute carrier family 30 (zinc transporter), member 4
• Berechnetes Molekulargewicht: 429 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC026089
• Gene symbol: ZNT4
• Gene ID (NCBI): 7782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZnT4, also known as SLC30A4, belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. ZnT4 transports Zn into the trans-Golgi apparatus for lactose synthesis, and across the apical cell membrane for efflux from MECs into milk. ZnT4 is expressed in numerous tissues and is enriched in the prostate. ZnT4 encodes a protein with a molecular weight of 47 kDa (PMID: 26538236).