SLC31A1 Rekombinanter Antikörper

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• KD/KO Validated

SLC31A1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240868D10

Kat-Nr. : 83844-5-RR

Synonyme

COPT1, 240868D10, CTR1, Truncated CTR1 form

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  WB analysis of HeLa using 83844-5-RR

  WB result of SLC31A1 antibody (83844-5-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

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  WB analysis of SW480 using 83844-5-RR

  WB result of SLC31A1 antibody (83844-5-RR; 1:800; room temperature for 1.5 hours) with wild-type and SLC31A1 knockout SW480 cells.

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  WB analysis using 83844-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83844-5-RR (SLC31A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83844-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83844-5-RR against Human SLC31A1 were performed. The affinity constant is 3.33 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, SW480-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83844-5-RR bindet in WB, ELISA SLC31A1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC31A1 fusion protein Ag26601
• Vollständiger Name: solute carrier family 31 (copper transporters), member 1
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21-23 kDa
• GenBank-Zugangsnummer: BC013611
• Gene symbol: SLC31A1
• Gene ID (NCBI): 1317
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC31A1, also known as CTR1, COPT1 and solute carrier family 31 member 1, is a high-affinity copper transporter found in the cell membrane of human cells. It plays a crucial role in maintaining copper homeostasis within the body and functions as a homotrimer to influence dietary copper absorption in the cell membrane (PMID: 37853210; 28507097). SLC31A1 has three putative transmembrane domains, with an N-terminus located extracellularly and a C-terminus located intracellularly (PMID: 12827356). SLC31A1 can be detected a a 28 kDa band corresponding to glycosylated hCTR1 monomer, and a 24 kDa band that is unglycosylated hCTR1. In some membrane preparations a C-terminal degradation product of 17 kDa is also seen (PMID: 16135512). Additionally, SLC31A1 may also resolve as a 30 to 35 kDa and a 60 to 70 kDa protein on immunoblots, forming oligomers corresponding to the expected size of a homotrimeric complex (PMID: 12827356).