SLC32A1/VGAT Polyklonaler Antikörper

SLC32A1/VGAT Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 14471-1-AP

Synonyme

SLC32A1, VGAT, GABA and glycine transporter, hVIAAT, Vesicular GABA transporter



Geprüfte Anwendungen

Erfolgreiche Detektion in WBungekochtes Maushirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHCRattenhirngewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PRattenhirngewebe, Maushirngewebe
Erfolgreiche Detektion in IF-FroRattenhirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14471-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA SLC32A1/VGAT und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Caenorhabditis elegans, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SLC32A1/VGAT fusion protein Ag5843
Vollständiger Name solute carrier family 32 (GABA vesicular transporter), member 1
Berechnetes Molekulargewicht 57 kDa
Beobachtetes Molekulargewicht 57 kDa
GenBank-ZugangsnummerBC053582
Gene symbol VGAT
Gene ID (NCBI) 140679
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SLC32A1/VGAT antibody 14471-1-APProtokoll herunterladen
IHC protocol for SLC32A1/VGAT antibody 14471-1-APProtokoll herunterladenl
IF protocol for SLC32A1/VGAT antibody 14471-1-APProtokoll herunterladen
IP protocol for SLC32A1/VGAT antibody 14471-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Acta Neuropathol Commun

Mutation-induced loss of APP function causes GABAergic depletion in recessive familial Alzheimer's disease: analysis of Osaka mutation-knockin mice.

Authors - Tomohiro Umeda
humanWB

Biochem J

Role of N-glycosylation in renal betaine transport.

Authors - Eva S Schweikhard
mouseWB

Biol Trace Elem Res

Effect of Voluntary Wheel Running on Anxiety- and Depression-Like Behaviors in Fluoride-Exposed Mice

Authors - Mengjie Qi
Caenorhabditis elegansIHC,IF

PLoS One

Mutant TDP-43 and FUS cause age-dependent paralysis and neurodegeneration in C. elegans.

Authors - Vaccaro Alexandra A
humanIF

Curr Issues Mol Biol

miR-92a-2-5p Regulates the Proliferation and Differentiation of ASD-Derived Neural Progenitor Cells.

Authors - Wenting Zhuang
mouseWB

Biol Trace Elem Res

Exercise Ameliorates Fluoride-induced Anxiety- and Depression-like Behavior in Mice: Role of GABA.

Authors - Qiqi Cao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Reyes (Verified Customer) (03-04-2024)

vGAT worked on my human brain FFPE sections although the quantification would be difficult due to the high autofluorescence

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
SLC32A1/VGAT Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:14471-1-AP)
FH

Mandi (Verified Customer) (03-10-2020)

Did not stain the nerve terminals well. Signal was localized to the soma mainly.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: human cortical neurons
FH

Ute (Verified Customer) (03-03-2020)

blocking solution 5% skimmed milk in 1x TBS-T

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse hippocampal lysat