VGAT Polyklonaler Antikörper

VGAT Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (2)

Kat-Nr. : 14471-1-AP

Synonyme

GABA and glycine transporter, hVIAAT, SLC32A1, Vesicular GABA transporter, VGAT, VIAAT

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14471-1-AP

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 14471-1-AP (VGAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14471-1-AP

  IP Result of anti-VGAT (IP:14471-1-AP, 4ug; Detection:14471-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of rat brain using 14471-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14471-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 14471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 14471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 14471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ungekochtes Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

14471-1-AP bindet in WB, IP, IHC, IF, ELISA VGAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Caenorhabditis elegans, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VGAT fusion protein Ag5843
• Vollständiger Name: solute carrier family 32 (GABA vesicular transporter), member 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC053582
• Gene symbol: SLC32A1
• Gene ID (NCBI): 140679
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.

Publikationen

Species	Application	Title
mouse	WB	Acta Neuropathol Commun; Mutation-induced loss of APP function causes GABAergic depletion in recessive familial Alzheimer's disease: analysis of Osaka mutation-knockin mice.; Authors - Tomohiro Umeda
human	WB	Biochem J; Role of N-glycosylation in renal betaine transport.; Authors - Eva S Schweikhard
Caenorhabditis elegans	IHC,IF	PLoS One; Mutant TDP-43 and FUS cause age-dependent paralysis and neurodegeneration in C. elegans.; Authors - Vaccaro Alexandra A
human	IF	Curr Issues Mol Biol; miR-92a-2-5p Regulates the Proliferation and Differentiation of ASD-Derived Neural Progenitor Cells.; Authors - Wenting Zhuang
mouse	WB	Biol Trace Elem Res; Exercise Ameliorates Fluoride-induced Anxiety- and Depression-like Behavior in Mice: Role of GABA.; Authors - Qiqi Cao
mouse	IF	Exp Anim; TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models; Authors - Haiqing Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mandi (Verified Customer) (03-10-2020)

Did not stain the nerve terminals well. Signal was localized to the soma mainly.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human cortical neurons

FH

Ute (Verified Customer) (03-03-2020)

blocking solution 5% skimmed milk in 1x TBS-T

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse hippocampal lysat