SLC35A3 Polyklonaler Antikörper

SLC35A3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 26874-1-AP

Synonyme

Golgi UDP GlcNAc transporter, NGT, SLC35A3

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  WB analysis using 26874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26874-1-AP (SLC35A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26874-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26874-1-AP (SLC35A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

26874-1-AP bindet in WB, ELISA SLC35A3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC35A3 fusion protein Ag25468
• Vollständiger Name: solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter), member A3
• Berechnetes Molekulargewicht: 325 aa, 36 kDa
• Beobachtetes Molekulargewicht: 32-36 and 41 kDa
• GenBank-Zugangsnummer: BC005136
• Gene symbol: SLC35A3
• Gene ID (NCBI): 23443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC35A3, also named as UDP-N-acetylglucosamine transporter(NGT), is located to the Golgi apparatus. SLC35A3 can form heterologous complexes with SLC35A2(UGT) and Mgats in the Golgi membrane which may promote biosynthesis of complex N-glycans. It has been reported that the mutation of SLC35A3 is associsted with skeletal dysplasia and epilepsies which could result from impaired glycosylation of proteins. SLC35A3 has some isoforms with 32-36 kDa and 41 kDa.(PMID: 25944901, 28777481  , 16344554, 23583405)

Publikationen

Species	Application	Title
human	WB	Sci Rep; Colorectal cancer with low SLC35A3 is associated with immune infiltrates and poor prognosis; Authors - Shuai Lu