SLC38A1 Polyklonaler Antikörper

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• KD/KO Validated

SLC38A1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28632-1-AP

Synonyme

Amino acid transporter A1, ATA1, NAT2, SAT1, SLC38A1, SNAT1

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  WB analysis using 28632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28632-1-AP (SLC38A1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28632-1-AP

  WB result of SLC38A1 antibody (28632-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC38A1 transfected Jurkat cells.

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  IHC staining of mouse brain using 28632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

28632-1-AP bindet in WB, IHC, ELISA SLC38A1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC38A1 fusion protein Ag30088
• Vollständiger Name: solute carrier family 38, member 1
• Berechnetes Molekulargewicht: 487 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa, 60-70 kDa
• GenBank-Zugangsnummer: BC010620
• Gene symbol: SLC38A1
• Gene ID (NCBI): 81539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Cell Signal; CENPA promotes glutamine metabolism and tumor progression by up-regulating SLC38A1 in endometrial cancer; Authors - Shuang Li
human	WB	Cancers (Basel); GluOC Induced SLC7A11 and SLC38A1 to Activate Redox Processes and Resist Ferroptosis in TNBC; Authors - Jiaojiao Xu ; KD Validated
human	WB,IHC	Cancer Lett; Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer; Authors - Minghua Bai