SLC38A3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC38A3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14315-1-PBS

Synonyme

---

• 

  WB analysis using 14315-1-AP (same clone as 14315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 14315-1-AP (same clone as 14315-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 14315-1-AP (same clone as 14315-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 14315-1-AP (same clone as 14315-1-PBS)

  IP result of anti-SLC38A3 (IP:14315-1-AP, 3ug; Detection:14315-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 14315-1-AP (same clone as 14315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 14315-1-AP (same clone as 14315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 14315-1-AP (same clone as 14315-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14315-1-AP (SLC38A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

14315-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SLC38A3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC38A3 fusion protein Ag5553
• Vollständiger Name: solute carrier family 38, member 3
• Berechnetes Molekulargewicht: 504 aa, 56 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC042875
• Gene symbol: SLC38A3
• Gene ID (NCBI): 10991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation.