SLC38A9 Rekombinanter Antikörper

SLC38A9 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240039E7

Kat-Nr. : 83182-5-RR

Synonyme

URLC11, Up-regulated in lung cancer 11, Solute carrier family 38 member 9, Neutral amino acid transporter 9, 240039E7

---

• 

  WB analysis of mouse pancreas using 83182-5-RR

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83182-5-RR (SLC38A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U2OS using 83182-5-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Slc38a9 Recombinant Antibody (83182-5-RR, Clone:240039E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 83182-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83182-5-RR against Human SLC38A9 were performed. The affinity constant is 0.715 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83182-5-RR bindet in WB, FC (Intra), ELISA SLC38A9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC38A9 fusion protein Ag15607
• Vollständiger Name: solute carrier family 38, member 9
• Berechnetes Molekulargewicht: 561 aa, 64 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC066891
• Gene symbol: SLC38A9
• Gene ID (NCBI): 153129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mTOR complex 1 (mTORC1) protein kinase is a master growth regulator that responds to multiple environmental cues. The lysosomal amino acid transporter SLC38A9 is a physical and functional component of this sensing complex. SLC38A9 (Solute Carrier Family 38 Member 9) was first identified as a potential amino acid transporter belonging to the slc38 family. SLC38A9 has some isoforms: 64,53,57 kDa.