SLC39A3 Monoklonaler Antikörper

SLC39A3 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11F6

Kat-Nr. : 68025-1-PBS

Synonyme

SLC39A3, Zinc transporter ZIP3, ZIP 3, ZIP3, Zrt and Irt like protein 3

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  WB analysis using 68025-1-Ig (same clone as 68025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68025-1-Ig (same clone as 68025-1-PBS)

  unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68025-1-Ig (same clone as 68025-1-PBS)

  37°C incubated A549 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68025-1-Ig (same clone as 68025-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 68025-1-Ig (same clone as 68025-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68025-1-Ig (same clone as 68025-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68025-1-PBS bindet in WB, Indirect ELISA SLC39A3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLC39A3 fusion protein Ag14289
• Vollständiger Name: solute carrier family 39 (zinc transporter), member 3
• Berechnetes Molekulargewicht: 314 aa, 34 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC005869
• Gene symbol: SLC39A3
• Gene ID (NCBI): 29985
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC39A3, also named as ZIP3, belongs to the ZIP transporter (TC 2.A.5) family. SLC39A3 is a Zn importer that acts as a zinc-influx transporter. SLC39A3 is highly expressed in a cell-type-specific manner in ovaries, testes, and the developing nervous system with limited expression in the small intestinal crypt cells, kidney glomerulus, and discrete regions of the liver. SLC39A3 is also expressed in highly specialized, hormonally responsive secretory tissues, such as the pancreas, prostate, and mammary gland (PMID: 19458277). SLC39A3 has 2 isoforms with the molecular mass of 11 and 34 kDa.