ZIP4 Polyklonaler Antikörper

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• KD/KO Validated

ZIP4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 20625-1-AP

Synonyme

AEZ, SLC39A4, Zinc transporter ZIP4, ZIP 4, ZIP4, Zrt and Irt like protein 4

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Galerie der Validierungsdaten

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  WB analysis of Colo320 cells using 20625-1-AP

  WB result of ZIP4 antibody (20625-1-AP, 1:2000) with si-control and si-ZIP4 transfected Colo320 cells.

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  WB analysis of human jejunum using 20625-1-AP

  human jejunum tissue were subjected to SDS PAGE followed by western blot with 20625-1-AP (ZIP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse pancreas using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Colo320 cells, humanes Jejunum-Gewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 12 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

20625-1-AP bindet in WB, IHC, IF, CoIP, ELISA ZIP4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZIP4 fusion protein Ag14524
• Vollständiger Name: solute carrier family 39 (zinc transporter), member 4
• Berechnetes Molekulargewicht: 647 aa, 68 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC062625
• Gene symbol: SLC39A4
• Gene ID (NCBI): 55630
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZIP4 is a zinc transporter encoded by the SLC39A4 gene, playing an important role in zinc homeostasis in mammalian cells. Recently, many studies have indicated that ZIP4 plays important roles in many kinds of carcinoma development and progression. ZIP4 may serve as a potential diagnostic and prognostic marker for gliomas and pancreatic cancer.

Publikationen

Species	Application	Title
human	WB	Gastroenterology; ZIP4 Increases Expression of Transcription Factor ZEB1 to Promote Integrin α3β1 Signaling and Inhibit Expression of the Gemcitabine Transporter ENT1 in Pancreatic Cancer Cells.; Authors - Mingyang Liu
human,mouse	WB,IHC	Gastroenterology; Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.; Authors - Xiuhui Shi ; KD Validated
human,mouse	WB,IHC,IF	Clin Cancer Res; ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.; Authors - Mingyang Liu ; KD Validated
human,mouse	IHC,WB	Cancer Lett; ZIP4 promotes non-small cell lung cancer metastasis by activating snail-N-cadherin signaling axis.; Authors - Yuanyuan Jiang
human	WB,IF	Int J Mol Sci; Zinc Favors Triple-Negative Breast Cancer's Microenvironment Modulation and Cell Plasticity; Authors - Marina Vogel-González
human	WB,IHC,CoIP	Cancers (Basel); ZIP4 Is a Novel Cancer Stem Cell Marker in High-Grade Serous Ovarian Cancer.; Authors - Qipeng Fan

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