SLC39A9 Polyklonaler Antikörper

SLC39A9 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17607-1-AP

Synonyme

SLC39A9, Zinc transporter ZIP9, ZIP 9, ZIP9, Zrt and Irt like protein 9

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  WB analysis using 17607-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17607-1-AP (SLC39A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 17607-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17607-1-AP (SLC39A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17607-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17607-1-AP (SLC39A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17607-1-AP bindet in WB, IHC, ELISA SLC39A9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC39A9 fusion protein Ag11752
• Vollständiger Name: solute carrier family 39 (zinc transporter), member 9
• Berechnetes Molekulargewicht: 307 aa, 32 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC047682
• Gene symbol: SLC39A9
• Gene ID (NCBI): 55334
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc transporter ZIP9 subfamily (SLC39A9) encodes a protein with characteristics of a membrane androgen receptor (mAR). Human ZIP9 is a 307-amino acid protein, which has eight putative transmembrane domains. ZIP9 has also been identified and characterized as a resident protein in the Golgi in DT40 and HeLa cell lines (PMID: 19420709, 23505453). ZIP9 may be present on the cell surface, as well as nuclear and mitochondrial membranes, in human breast and prostate cancer cell lines, where it mediates testosterone induction of apoptosis (PMID: 29952128). knockout of ZIP9 would lead to zinc accumulation in the secretory pathway, which could influence the general ion homeostasis and in turn the glycosylation outcome (PMID: 36648436).