SLC3A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC3A1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 16343-1-AP

Synonyme

NBAT, D2H, CSNU1, b(0,+)-type amino acid transporter-related heavy chain, ATR1

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  WB analysis using 16343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16343-1-AP (SLC3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16343-1-AP

  IP result of anti-SLC3A1 (IP:16343-1-AP, 4ug; Detection:16343-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 16343-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16343-1-AP (SLC3A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16343-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16343-1-AP (SLC3A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16343-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16343-1-AP (SLC3A1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 16343-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16343-1-AP (SLC3A1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Produktinformation

16343-1-AP bindet in WB, IHC, IF, IP, ELISA SLC3A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC3A1 fusion protein Ag9525
• Vollständiger Name: solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
• Berechnetes Molekulargewicht: 685 aa, 79 kDa
• Beobachtetes Molekulargewicht: 78-90 kDa
• GenBank-Zugangsnummer: BC022386
• Gene symbol: SLC3A1
• Gene ID (NCBI): 6519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney.; Authors - M Takasato
mouse	WB	Nat Commun; Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidney.; Authors - Joseph O Lamontagne
mouse	IF	Dev Cell; AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.; Authors - Alexander G Marneros
human	WB	Theranostics; Cysteine transporter SLC3A1 promotes breast cancer tumorigenesis.; Authors - Yang Jiang ; KD Validated
mouse	IF	Sci Rep; Kidney-specific transposon-mediated gene transfer in vivo.; Authors - Lauren E Woodard
mouse	WB	FASEB J; Epidermal growth factor deficiency predisposes to progressive renal disease.; Authors - Alia M Zeid

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Felisha (Verified Customer) (01-03-2020)

The primary antibody worked great to localize the derived human stem cells in the mouse tissue samples that were sectioned and paraffin embedded. Wonderful for immunohistochemistry!

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1: 200
• Cell Tissue Type: Mouse tissue and human cells