CD98/SLC3A2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD98/SLC3A2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

30

Kat-Nr. : 15193-1-AP

Synonyme

4F2, 4F2 heavy chain antigen, 4F2HC, 4T2HC, CD98, CD98/SLC3A2, CD98HC, MDU1, NACAE, SLC3A2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15193-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15193-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15193-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98/SLC3A2 antibody (15193-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 15193-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD98/SLC3A2 antibody (15193-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15193-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human CD98/SLC3A2 (15193-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, humanes Plazenta-Gewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, A549-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

15193-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CD98/SLC3A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD98/SLC3A2 fusion protein Ag7280
• Vollständiger Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 85-94 kDa, 120-130 kDa
• GenBank-Zugangsnummer: BC001061
• Gene symbol: SLC3A2
• Gene ID (NCBI): 6520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Publikationen

Species	Application	Title
human	WB	ACS Nano; Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy; Authors - Rangrang Fan
human	WB,IHC	Sci Transl Med; Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.; Authors - Kana Hasegawa
mouse	IF	Nat Commun; Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.; Authors - Weiwei Yu
human	IF	J Allergy Clin Immunol; Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function.; Authors - Siddhartha S Saha
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai
mouse	WB	Cell Rep; Brown adipocyte ATF4 activation improves thermoregulation and systemic metabolism; Authors - Esther Paulo