CD98/SLC3A2 Polyklonaler Antikörper

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• KD/KO Validated

CD98/SLC3A2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(60)

Kat-Nr. : 15193-1-AP

Synonyme

CD98, SLC3A2, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2, MDU1

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  WB analysis using 15193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15193-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15193-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98/SLC3A2 antibody (15193-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, humanes Plazenta-Gewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 49 publications below
• IHC: See 10 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Produktinformation

15193-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CD98/SLC3A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD98/SLC3A2 fusion protein Ag7280
• Vollständiger Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 85-94 kDa, 120-130 kDa
• GenBank-Zugangsnummer: BC001061
• Gene symbol: CD98
• Gene ID (NCBI): 6520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Publikationen

Species	Application	Title
human	WB	ACS Nano; Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy; Authors - Rangrang Fan
human	WB,IHC	Sci Transl Med; Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.; Authors - Kana Hasegawa
mouse	IF	Nat Commun; Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.; Authors - Weiwei Yu
human	IF	J Allergy Clin Immunol; Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function.; Authors - Siddhartha S Saha
mouse,human	WB,IHC,IF	JACC Basic Transl Sci; CircBTBD7-420aa Encoded by hsa_circ_0000563 Regulates the Progression of Atherosclerosis and Construction of circBTBD7-420aa Engineered Exosomes; Authors - Xiongkang Gan
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai