SLC5A6 Polyklonaler Antikörper

SLC5A6 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26407-1-AP

Synonyme

Na(+)-dependent multivitamin transporter, SMVT, Sodium-dependent multivitamin transporter

---

• 

  WB analysis of Caco-2 using 26407-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 26407-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 26407-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 26407-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 26407-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 26407-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 26407-1-AP

  IP result of anti-SLC5A6 (IP:26407-1-AP, 4ug; Detection:26407-1-AP 1:500) with mouse brain tissue lysate 5200ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, COLO 320-Zellen, Maushirngewebe, Maus-Dünndarmgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

26407-1-AP bindet in WB, IP, ELISA SLC5A6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC5A6 fusion protein Ag23907
• Vollständiger Name: solute carrier family 5 (sodium-dependent vitamin transporter), member 6
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC012806
• Gene symbol: SLC5A6
• Gene ID (NCBI): 8884
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC5A6, also known as the sodium-dependent multivitamin transporter (hSMVT), is a transmembrane protein that plays a crucial role in the uptake of essential vitamins, including biotin, pantothenic acid, and lipoic acid. This protein is widely expressed in various human tissues, including the placenta, intestine, brain, liver, lung, kidney, cornea, retina, and heart. It uses energy from the transmembrane sodium ion gradient and membrane potential to actively transport these vitamins into cells. Due to different glycosylation modifications, SLC5A6 may exhibit two bands at ~55 kDa and ~69 kDa. (PMID: 20980265, PMID: 25971966)

Publikationen

Species	Application	Title
mouse,human	WB	Nat Metab; Vitamin B5 supports MYC oncogenic metabolism and tumor progression in breast cancer; Authors - Peter Kreuzaler
human	WB	Mol Cell Proteomics; Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.; Authors - James R Byrnes