GAT1 Rekombinanter Antikörper

GAT1 Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242637D11

Kat-Nr. : 85060-2-PBS

Synonyme

SLC6A1, 242637D11, GABA Transporter 1, GABATHG, GABATR

---

• 

  WB analysis using 85060-2-RR (same clone as 85060-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85060-2-RR (SLC6A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85060-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01787-2

  Sandwich ELISA standard curve of MP01787-2, Human GAT1 Recombinant Matched Antibody Pair - PBS only. 85060-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29712. 85060-1-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

• 

  Sample test of MP01787-2

  The mean GAT1 concentration was determined to be 12.15 ng/mL in SH-SY5Y cell extract based on a 1.40 mg/mL extract load and 1,095.09 ng/mL in mouse cerebellum tissue extract based on a 6.00 mg/mL extract load.

• 

  IHC staining of mouse cerebellum using 85060-2-RR (same clone as 85060-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85060-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 85060-2-RR (same clone as 85060-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85060-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 85060-2-RR (same clone as 85060-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85060-2-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 85060-2-RR (same clone as 85060-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GAT1 antibody (85060-2-RR, Clone: 242637D11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85060-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01787-2

  Cytometric bead array standard curve of MP01787-2, GAT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85060-2-PBS. Detection antibody: 85060-1-PBS. Standard: Ag29712. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 85060-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85060-2-RR against Human GAT1 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

85060-2-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test GAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GAT1 fusion protein Ag29712
• Vollständiger Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC033904
• Gene symbol: GABA Transporter 1/GAT1
• Gene ID (NCBI): 6529
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.