• Featured Product
  • KD/KO Validated

Serotonin transporter Polyklonaler Antikörper

Serotonin transporter Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 19559-1-AP

Synonyme

SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT



Geprüfte Anwendungen

Erfolgreiche Detektion in WBSH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IF-PMaus-Cerebellum-Gewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunfluoreszenz (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19559-1-AP bindet in WB, IHC, IF-P, ELISA Serotonin transporter und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Berechnetes Molekulargewicht 70 kDa
Beobachtetes Molekulargewicht70 kDa and 140 kDa
GenBank-ZugangsnummerNM_001045
Gene symbol Serotonin transporter
Gene ID (NCBI) 6532
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319). This antibody detected 70 kDa and 140 kDa bands in SDS-PAGE. The 140 kDa band corresponds to the dimer of high mannose-type SERT (PMID: 19323988).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Serotonin transporter antibody 19559-1-APProtokoll herunterladen
IF protocol for Serotonin transporter antibody 19559-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress.

Authors - Jane L Tarry-Adkins
mouseWB

Sci Rep

Sepsis-induced elevation in plasma serotonin facilitates endothelial hyperpermeability.

Authors - Yicong Li
  • KO Validated
mouseWB

J Cell Physiol

Serotonin Transporter Protects the Placental Cells Against Apoptosis in Caspase 3-Independent Pathway.

Authors - Coedy Hadden
  • KO Validated
mouseWB

Heliyon

Comparison of CUMS at different pregnancy stages, maternal separation, and their effects on offspring in postpartum depression mouse models

Authors - Fei Fei
mouse,humanWB,IHC

Cell Rep

SSRI antidepressant citalopram reverses the Warburg effect to inhibit hepatocellular carcinoma by directly targeting GLUT1

Authors - Fangyuan Dong
mouseIHC

Eur J Neurosci

The alteration of serotonergic markers in the amygdala and raphe nuclei of oestrogen receptor β knock-out female mice

Authors - Daniel Kalinowski