SLC6A8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC6A8 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20299-1-PBS

Synonyme

Sodium- and chloride-dependent creatine transporter 1, CT1, CRTR, CRT, Creatine transporter 1

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  WB analysis using 20299-1-AP (same clone as 20299-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20299-1-AP (SLC6A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 20299-1-AP (same clone as 20299-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 20299-1-AP (SLC6A8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20299-1-AP (SLC6A8 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20299-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA SLC6A8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC6A8 fusion protein Ag14110
• Vollständiger Name: solute carrier family 6 (neurotransmitter transporter, creatine), member 8
• Berechnetes Molekulargewicht: 635 aa, 71 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC012355
• Gene symbol: SLC6A8
• Gene ID (NCBI): 6535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC6A8, also known as the sodium- and chloride-dependent creatine transporter 1 (CT1), plays a critical role in transporting creatine, a crucial molecule for energy metabolism, into cells. SLC6A8 belongs to the solute carrier family 6 (SLC6), responsible for transporting diverse molecules across cell membranes. SLC6A8 expression is highest in muscle, kidney, and other tissues with high energy demands. Mutations in SLC6A8 cause creatine transporter deficiency, an X-linked mental retardation disorder (PMID: 17465020).