SLC7A11/xCT Polyklonaler Antikörper

SLC7A11/xCT Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 32384-1-AP

Synonyme

Amino acid transport system xc-, Cystine/glutamate transporter, Slc7a11, Solute carrier family 7 member 11, Subtle gray protein

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  WB analysis using 32384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32384-1-AP (SLC7A11/xCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ungekochte HeLa-Zellen, ungekochte HepG2-Zellen, ungekochtes Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

32384-1-AP bindet in WB, IF, ELISA SLC7A11/xCT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC7A11/xCT fusion protein Ag38215
• Vollständiger Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 11
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: NM_011990
• Gene symbol: Slc7a11
• Gene ID (NCBI): 26570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC7A11 (also known as xCT) is a membrane transporter involved in cysteine uptake. It promotes glutathione synthesis through cystine uptake and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers, including lung cancer. 32384-1-AP recognizes the 35-40 kDa protein similar to the paper published (PMID: 36747082).

Publikationen

Species	Application	Title
mouse	IF	Toxicology; Chronic exposure to polystyrene microplastics induces renal fibrosis via ferroptosis; Authors - Runyang Hong