SLC7A5 Rekombinanter Antikörper

SLC7A5 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241476D5

Kat-Nr. : 84178-5-RR

Synonyme

LAT1, 241476D5, 4F2 LC, 4F2 light chain, 4F2LC

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  WB analysis of A549 using 84178-5-RR

  A549 cells were subjected to SDS PAGE followed by western blot with 84178-5-RR (SLC7A5 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 84178-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84178-5-RR (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84178-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84178-5-RR (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-29 using 84178-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using SLC7A5 antibody (84178-5-RR, Clone: 241476D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HT-29 using 84178-5-RR

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug SLC7A5 Recombinant antibody (84178-5-RR, Clone:241476D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84178-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84178-5-RR against Human SLC7A5 were performed. The affinity constant is 0.319 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, MCF-7-Zellen, MKN-45-Zellen, SW480-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen
• Erfolgreiche Detektion in FC (Intra): HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84178-5-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA SLC7A5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC7A5 fusion protein Ag29164
• Vollständiger Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Berechnetes Molekulargewicht: 507 aa, 55 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC039692
• Gene symbol: SLC7A5
• Gene ID (NCBI): 8140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)