SLC9A3/NHE3 Polyklonaler Antikörper

SLC9A3/NHE3 Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33559-1-AP

Synonyme

NHE3, NHE-3, SLC9A3

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  WB analysis using 33559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33559-1-AP (SLC9A3/NHE3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse kidney using 33559-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SLC9A3/NHE3 antibody (33559-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 33559-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SLC9A3/NHE3 antibody (33559-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bei 37°C inkubiertes Mausnierengewebe
• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe, Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

33559-1-AP bindet in WB, IF-P, ELISA SLC9A3/NHE3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC9A3/NHE3 fusion protein Ag38302
• Vollständiger Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3
• Berechnetes Molekulargewicht: 834 aa, 93 kDa
• Beobachtetes Molekulargewicht: 80-100 kDa
• GenBank-Zugangsnummer: BC101669
• Gene symbol: NHE3
• Gene ID (NCBI): 6550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC9A3, also known as NHE3, belongs to the sodium hydrogen exchange protein family (NHE) and is mainly responsible for regulating the concentration of sodium and hydrogen ions inside and outside the cell. SLC9A3 is a Major apical Na+/H+ exchanger in kidney and intestine, playing an important role in renal and intestine Na+ absorption and blood pressure regulation (PMID:24622516, 2635877). Mutations in SLC9A3 cause Congenital Sodium Diarrhea (PMID: 26358773, 31276831).