SLC9A3R2 Rekombinanter Antikörper

SLC9A3R2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241215A3

Kat-Nr. : 84060-5-RR

Synonyme

241215A3, E3KARP, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHE3RF2, NHERF 2

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  WB analysis using 84060-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84060-5-RR (SLC9A3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84060-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84060-5-RR against Human SLC9A3R2 were performed. The affinity constant is 0.476 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: T-47D-Zellen, HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84060-5-RR bindet in WB, ELISA SLC9A3R2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC9A3R2 fusion protein Ag13476
• Vollständiger Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
• Berechnetes Molekulargewicht: 337 aa, 37 kDa
• Beobachtetes Molekulargewicht: ~45 kDa
• GenBank-Zugangsnummer: BC106001
• Gene symbol: SLC9A3R2
• Gene ID (NCBI): 9351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)