NHE8 Polyklonaler Antikörper

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• KD/KO Validated

NHE8 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18318-1-AP

Synonyme

KIAA0939, Na(+)/H(+) exchanger 8, NHE 8, NHE8, SLC9A8, Sodium/hydrogen exchanger 8

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  WB analysis using 18318-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18318-1-AP

  WB result of NHE8 antibody (18318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NHE8 transfected HEK-293 cells.

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  WB analysis of mouse liver using 18318-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18318-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 18318-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 18318-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 18318-1-AP (NHE8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maus-Kolongewebe, Mausnierengewebe, Mauslebergewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolongewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Hodengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

18318-1-AP bindet in WB, IHC, IF/ICC, ELISA NHE8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NHE8 fusion protein Ag13111
• Vollständiger Name: solute carrier family 9 (sodium/hydrogen exchanger), member 8
• Berechnetes Molekulargewicht: 581 aa, 65 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC112213
• Gene symbol: SLC9A8
• Gene ID (NCBI): 23315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NHE8, encoded by SLC9A8, is a member of sodium/hydrogen exchanger family which are transmembrane proteins that mediate the electroneutral exchange of sodium (Na) for hydrogen (H) and get involved in intracellular pH homeostasis, cell volume regulation, acid-base regulation, and so on. NHE8 has a broad tissue distribution, with relatively high abundance in the gastrointestinal tract. It has been reported that NHE8 expression differs among different regions in the stomach, very low in nonglandular region whereas very high in glandular region. The predicted molecular weight of NHE8 is 64-65 kDa, while its glycosylated form often migrates around 70-85 kDa.

Publikationen

Species	Application	Title
human	WB,IF	bioRxiv; Virus-induced vesicular acidification enhances HIV immune evasion; Authors - Marianne E Yaple-Maresh