SLCO1B3/OATP1B3 Monoklonaler Antikörper

SLCO1B3/OATP1B3 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D9A4

Kat-Nr. : 66381-1-PBS

Synonyme

OATP 8, OATP1B3, OATP8, SLCO1B3, 1D9A4

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  WB analysis of HepG2 using 66381-1-Ig (same clone as 66381-1-PBS)

  Western blot analysis of SLCO1B3/OATP1B3 in various cell lines using Proteintech antibody 66381-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66381-1-Ig (same clone as 66381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3/OATP1B3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66381-1-Ig (same clone as 66381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66381-1-Ig (same clone as 66381-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLCO1B3/OATP1B3 antibody (66381-1-Ig, Clone: 1D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66381-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SLCO1B3/OATP1B3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLCO1B3/OATP1B3 fusion protein Ag19033
• Vollständiger Name: solute carrier organic anion transporter family, member 1B3
• Berechnetes Molekulargewicht: 702 aa, 77 kDa
• Beobachtetes Molekulargewicht: 68-77 kDa
• GenBank-Zugangsnummer: BC105597
• Gene symbol: SLCO1B3
• Gene ID (NCBI): 28234
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLCO1B3, also known as OATP8 or OATP1B3, is a member of Organic Anion Transporting Polypeptides (OATPs) which are sodium-independent organic anion transporters mediating the uptake of a wide range of structurally diverse endogenous and exogenous compounds including bile acids, hormone conjugates, peptides, toxins, as well as a multitude of therapeutic drugs. SLCO1B3 is specifically expressed in liver under normal condition, while its expression has also been observed in cancer tissues like colon, prostate and pancreas. It corresponds to Oatp1b2 in mice, with the gene symbol Slco1b2. The mouse genome encodes only a single transporter gene in the OATP1B subfamily, because SLCO1B1 and SLCO1B3 arose in primates by gene duplication after divergence from rodents. The molecular mass of SLCO1B3 is highly dependent on the glycosylation modification (deglycosylated form of 65 kDa, fully-glycosylated form of 120 kDa).