PGT Polyklonaler Antikörper

PGT Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14327-1-AP

Synonyme

SLCO2A1, PHOAR2, OATP2A1

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  WB analysis using 14327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14327-1-AP (PGT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14327-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14327-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14327-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14327-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14327-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14327-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 14327-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14327-1-AP (PGT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

14327-1-AP bindet in WB, IHC, ELISA PGT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGT fusion protein Ag5594
• Vollständiger Name: solute carrier organic anion transporter family, member 2A1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC041140
• Gene symbol: PGT
• Gene ID (NCBI): 6578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (8787677). This antibody is expected to detect the endogenous PGT protein.

Publikationen

Species	Application	Title
pig	IHC	Biol Reprod; Prostaglandin Transporters ABCC4 and SLCO2A1 in the Uterine Endometrium and Conceptus During Pregnancy in Pigs.; Authors - Heewon Seo
mouse	WB	Int Immunopharmacol; Effect of oridonin on oxylipins in the livers of mice with acute liver injury induced by D-galactosamine and lipopolysaccharide.; Authors - Zhikun Zhan
human	IHC	Dig Dis Sci; Four Variants of SLCO2A1 Identified in Three Chinese Patients with Chronic Enteropathy Associated with the SLCO2A1 Gene.; Authors - Hui Huang