SLD5 Polyklonaler Antikörper

SLD5 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13315-1-AP

Synonyme

GINS4, GINS complex subunit 4, DNA replication complex GINS protein SLD5, N-terminally processed

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  WB analysis of mouse thymus using 13315-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13315-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 13315-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SLD5 antibody (13315-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13315-1-AP bindet in WB, IF/ICC, ELISA SLD5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLD5 fusion protein Ag4158
• Vollständiger Name: GINS complex subunit 4 (Sld5 homolog)
• Berechnetes Molekulargewicht: 223 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC027454
• Gene symbol: SLD5
• Gene ID (NCBI): 84296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLD5 (DNA replication complex GINS protein SLD5) is also named as GINS4 (GINS complex subunit 4). SLD5 is required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PMID: 17417653). SLD5 forms a GINS complex with PSF1, PSF2 and PSF3, which is essential for the initiation of DNA replication in lower eukaryotes (PMID: 24244394). SLD5 is a member of the GINS complex, essential for DNA replication in eukaryotes. It has been reported that SLD5 is involved in early embryogenesis in the mouse, and cell cycle progression and genome integrity in Drosophila (PMID: 27499248).