SLIT2-Specific Rekombinanter Antikörper

SLIT2-Specific Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250648A6

Kat-Nr. : 82725-4-PBS

Synonyme

SLIT2, SLIL3, Slit 2, Slit homolog 2 protein, Slit homolog 2 protein C-product

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  WB analysis of HEK-293 using 82725-4-RR (same clone as 82725-4-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82725-4-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 82725-4-RR (same clone as 82725-4-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82725-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 82725-4-RR (same clone as 82725-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82725-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 82725-4-RR (same clone as 82725-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82725-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82725-4-PBS bindet in WB, IHC, Indirect ELISA SLIT2-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: slit homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 170 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_004787
• Gene symbol: SLIT2
• Gene ID (NCBI): 9353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLIT2, also named as SLIL3, is thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development it is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2.