SLIT2-Specific Rekombinanter Antikörper

SLIT2-Specific Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250648A6

Kat-Nr. : 82725-4-RR

Synonyme

SLIT2, SLIL3, Slit 2, Slit homolog 2 protein, Slit homolog 2 protein C-product

---

• 

  WB analysis of HEK-293 using 82725-4-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 82725-4-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 82725-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 82725-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82725-4-RR bindet in WB, IHC, ELISA SLIT2-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: slit homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 170 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_004787
• Gene symbol: SLIT2
• Gene ID (NCBI): 9353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLIT2, also named as SLIL3, is thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development it is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2.