SLITRK3 Polyklonaler Antikörper

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• KD/KO Validated

SLITRK3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21649-1-AP

Synonyme

SLIT and NTRK-like protein 3, KIAA0848

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  WB analysis of mouse brain using 21649-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21649-1-AP

  IP result of anti-SLITRK3 (IP:21649-1-AP, 4ug; Detection:21649-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse intestine using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse intestine using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Ratten-Dünndarmgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21649-1-AP bindet in WB, IHC, IF, IP, ELISA SLITRK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLITRK3 fusion protein Ag16352
• Vollständiger Name: SLIT and NTRK-like family, member 3
• Berechnetes Molekulargewicht: 977 aa, 109 kDa
• Beobachtetes Molekulargewicht: 120-125 kDa
• GenBank-Zugangsnummer: BC114621
• Gene symbol: SLITRK3
• Gene ID (NCBI): 22865
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB, IHC	Neuron; Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.; Authors - Jun Li
rat	WB	Proc Natl Acad Sci U S A; Slitrks control excitatory and inhibitory synapse formation with LAR receptor protein tyrosine phosphatases.; Authors - Yim Yeong Shin YS ; KD Validated
human	WB,IF	Mol Biomed; Role of novel cancer gene SLITRK3 to activate NTRK3 in squamous cell lung cancer.; Authors - Aliccia Bollig-Fischer ; KD Validated
human	WB	Front Mol Neurosci; Human mutations in SLITRK3 implicated in GABAergic synapse development in mice; Authors - Stephanie Efthymiou
mouse	WB	J Neurosci; A critical role of Neuroligin 2 C-terminus in OCD and social behavior; Authors - Saurabh Pandey