SLPI Polyklonaler Antikörper

SLPI Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32205-1-AP

Synonyme

ALK1, ALP, Antileukoprotease, Antileukoproteinase, HUSI-1

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  WB analysis using 32205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32205-1-AP (SLPI antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 32205-1-AP

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:800) with HeLa cells lysate 1200 ug.

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  IP experiment of HeLa using 32205-1-AP

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:800) with HeLa cells lysate 1200 ug.

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  IP experiment of human saliva using 32205-1-AP

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:1000) with human saliva lysate 400 ug.

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  IF Staining of HeLa using 32205-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SLPI antibody (32205-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, human saliva
• Erfolgreiche IP: HeLa-Zellen, human saliva
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32205-1-AP bindet in WB, IF/ICC, IP, ELISA SLPI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLPI fusion protein Ag36523
• Vollständiger Name: secretory leukocyte peptidase inhibitor
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC020708
• Gene symbol: SLPI
• Gene ID (NCBI): 6590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Secretory leukocyte protease inhibitor (SLPI) is also named as Antileukoproteinase (ALP), BLPI, HUSI-1, WAP4, WFDC4 and mucus proteinase inhibitor (MPI). The SLPI is a multifunctional protein involved in the modulation of immunological response and the inhibition of protease activities. SLPI acts as an inhibitor of proteases, exerts antibacterial properties, and suppresses the transcription of proinflammatory genes through the nuclear factor-kappa B (NF-κB) pathway (PMID: 37356220). SLPI is as a serine protease inhibitor and also as a critical mediator that is involved in the PTH-mediated shift to the osteoblastic phase (PMID: 17474882). Slpi induction in osteoblasts enhances its differentiation, and increases osteoblast-osteoclast contact, thereby suppressing osteoclastic function (PMID: 17474882). SLPI is an evolutionarily conserved, pleiotropic protein expressed at mucosal surfaces, mainly by epithelial cells (PMID: 33602652). SLPI maintains homeostasis at barrier tissues by preventing tissue destruction and regulating the threshold of inflammatory immune responses, while protecting the host from infection. However, as recent studies demonstrate that overexpression of SLPI increases the metastatic potential of epithelial tumors (PMID: 33602652). The role of this protein as a regulatory agent has been implicated in various types of cancer (PMID: 37356220).