SLPI Rekombinanter Antikörper

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• KD/KO Validated

SLPI Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241057F8

Kat-Nr. : 83965-4-RR

Synonyme

241057F8, ALK1, ALP, Antileukoprotease, Antileukoproteinase

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• 

  WB analysis of human saliva using 83965-4-RR

  human saliva were subjected to SDS PAGE followed by western blot with 83965-4-RR (SLPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 83965-4-RR

  WB result of SLPI antibody (83965-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLPI transfected PC-3 cells.

• 

  IHC staining of human cervical cancer using 83965-4-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83965-4-RR (SLPI antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 83965-4-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83965-4-RR (SLPI antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83965-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83965-4-RR against Human SLPI were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human saliva, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83965-4-RR bindet in WB, IHC, ELISA SLPI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLPI fusion protein Ag1673
• Vollständiger Name: secretory leukocyte peptidase inhibitor
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC020708
• Gene symbol: SLPI
• Gene ID (NCBI): 6590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLPI, a member of Whey acidic protein family, could mediate a variety of biological processes including cell proliferation and apoptosis, repairing reaction and immune response, with crucial roles in anti-protease, anti-inflammatory, anti-bacterial and anti-virus. SLPI is mainly expressed in epithelial cells.