SLTM Polyklonaler Antikörper

SLTM Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Rezensionen (2)

Kat-Nr. : 17889-1-AP

Synonyme

SAFB-like transcription modulator, Modulator of estrogen-induced transcription, MET

---

• 

  WB analysis of mouse brain using 17889-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17889-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17889-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 17889-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17889-1-AP (SLTM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17889-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLTM antibody (17889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17889-1-AP bindet in WB, IHC, IF/ICC, ELISA SLTM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLTM fusion protein Ag12060
• Vollständiger Name: SAFB-like, transcription modulator
• Berechnetes Molekulargewicht: 478aa,57 kDa; 1034aa,117 kDa
• Beobachtetes Molekulargewicht: 135-140 kDa
• GenBank-Zugangsnummer: BC046119
• Gene symbol: SLTM
• Gene ID (NCBI): 79811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLTM, also named as Modulator of estrogen-induced transcription, is a 1034 amino acid protein, which localizes in the nucleus. When it is overexpressed, SLTM acts as a general inhibitor of transcription that eventually leads to apoptosis. The calculated molecular weight of SLTM is 117 kDa, but modified SLTM is about 135-140 kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tsimafei (Verified Customer) (08-30-2025)

Specific Sltm detection in human cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

SLTM Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:17889-1-AP)

FH

Aleksandr (Verified Customer) (07-29-2025)

Excellent antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: RKO

SLTM Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:17889-1-AP)