SLU7 Polyklonaler Antikörper

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• KD/KO Validated

SLU7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, RIP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12050-1-AP

Synonyme

9G8, hSlu7, Pre mRNA splicing factor SLU7, SLU7

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  WB analysis of Jurkat using 12050-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12050-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12050-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12050-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12050-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12050-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12050-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12050-1-AP (SLU7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, K-562-Zellen, Maus-Kolongewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Kolonkarzinomgewebe, Ratten-Pankreasgewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

12050-1-AP bindet in WB, IF, RIP, IHC, ELISA SLU7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLU7 fusion protein Ag2680
• Vollständiger Name: SLU7 splicing factor homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 586 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC010634
• Gene symbol: SLU7
• Gene ID (NCBI): 10569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pre-mRNA splicing occurs in two sequential transesterification steps. SLU7 is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions.

Publikationen

Species	Application	Title
human	RIP	Mol Cancer; A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer; Authors - Runjie Song
human,mouse	WB,IHC,IF	Adv Sci (Weinh); G3BP1 and SLU7 Jointly Promote Immune Evasion by Downregulating MHC-I via PI3K/Akt Activation in Bladder Cancer; Authors - Xianchong Zheng ; KD Validated