SMAD1 Monoklonaler Antikörper

SMAD1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D10B8

Kat-Nr. : 66559-1-PBS

Synonyme

2D10B8, BSP1, BSP-1, hSMAD1, JV41

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  WB analysis using 66559-1-Ig (same clone as 66559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66559-1-Ig (SMAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66559-1-Ig (same clone as 66559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66559-1-Ig (SMAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66559-1-Ig (same clone as 66559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66559-1-Ig (SMAD1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66559-1-Ig (same clone as 66559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66559-1-Ig (SMAD1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66559-1-PBS bindet in WB, IHC, Indirect ELISA SMAD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMAD1 fusion protein Ag0701
• Vollständiger Name: SMAD family member 1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC001878
• Gene symbol: SMAD1
• Gene ID (NCBI): 4086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase