SMAD2 Rekombinanter Antikörper

SMAD2 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240950G10

Kat-Nr. : 83841-3-PBS

Synonyme

hMAD 2, hMAD-2, hSMAD2, JV18, JV18 1

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  Sandwich ELISA standard curve of MP00791-1

  Sandwich ELISA standard curve of MP00791-1, Human SMAD2 Recombinant Matched Antibody Pair - PBS only. 83841-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3237. 83841-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00791-1

  Serum of sixteen individual healthy human donors was measured. The human SMAD2 concentration of detected samples was determined to be 2.17 ng/mL with a range of 0.34 - 8.22 ng/mL.

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  Sample test of MP00791-1

  HEK-293T, HeLa, HepG2 and MCF-7 cell lysates were measured. The human SMAD2 concentration of detected samples was determined to be 43.85 ng/mL in HEK-293T cell lysate (based on a 5.5 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 33.98 ng/mL in HeLa cell lysate (based on a 7 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 24.50 ng/mL in HepG2 cell lysate (based on a 4.0 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 12.02 ng/mL in MCF-7 cell lysate (based on a 3.1 mg/mL extract load).

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  Cytometric bead array standard curve of MP00791-1

  Cytometric bead array standard curve of MP00791-1, SMAD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83841-2-PBS. Detection antibody: 83841-3-PBS. Standard: Ag3237. Range: 0.469-60 ng/mL.

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Geprüfte Anwendungen

Produktinformation

83841-3-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test SMAD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SMAD2 fusion protein Ag3237
• Vollständiger Name: SMAD family member 2
• Berechnetes Molekulargewicht: 467 aa, 52 kDa
• GenBank-Zugangsnummer: BC014840
• Gene symbol: SMAD2
• Gene ID (NCBI): 4087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.