SMAD3 Polyklonaler Antikörper

SMAD3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30130-1-PBS

Synonyme

hMAD 3, hMAD-3, hSMAD3, HSPC193, HsT17436

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  WB analysis using 30130-1-AP (same clone as 30130-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30130-1-AP (SMAD3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 30130-1-AP (same clone as 30130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30130-1-AP (same clone as 30130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30130-1-AP (same clone as 30130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 30130-1-AP (same clone as 30130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30130-1-PBS bindet in WB, IHC, Indirect ELISA SMAD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMAD3 fusion protein Ag32595
• Vollständiger Name: SMAD family member 3
• Berechnetes Molekulargewicht: 48kd
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: NM_005902
• Gene symbol: SMAD3
• Gene ID (NCBI): 4088
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.