SMAD3 Monoklonaler Antikörper

SMAD3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(121)

CloneNo.

1F10B2

Rezensionen (1)

Kat-Nr. : 66516-1-Ig

Synonyme

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

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• 

  WB analysis using 66516-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66516-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 66516-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66516-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, 4T1-Zellen, A431-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 113 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below

Produktinformation

66516-1-Ig bindet in WB, IHC, IF, IP, CoIP, ChIP, ELISA SMAD3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SMAD family member 3
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: NM_005902
• Gene symbol: SMAD3
• Gene ID (NCBI): 4088
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu
human	WB	Cancer Lett; Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma; Authors - Qianyu Shi
human,mouse	WB	Cell Chem Biol; Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction; Authors - Wanqi Yang
mouse	WB,IF	Cell Mol Life Sci; Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion; Authors - Shiju Ye
human	CoIP	Diabetes; Overexpressed Poldip2 incurs retinal fibrosis via the TGFβ1/SMAD3 signaling pathway in diabetic retinopathy; Authors - Zhiyu Ji

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Juliette (Verified Customer) (11-25-2024)

Good

• Applications: Immunofluorescence
• Cell Tissue Type: Human