SMAD5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMAD5 Polyklonal Antikörper für FC, IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 12167-1-AP

Synonyme

Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 12167-1-AP

  WB result of SMAD5 antibody (12167-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD5 transfected K-562 cells.

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  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12167-1-AP

  IP Result of anti-SMAD5 (IP:12167-1-AP, 4ug; Detection:12167-1-AP 1:1000) with K-562 cells lysate 3200ug.

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  IF Staining of MCF-7 using 12167-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody 12167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of MCF-7 using 12167-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug SMAD5 antibody (12167-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 18 publications below
• FC: See 1 publications below

Produktinformation

12167-1-AP bindet in WB, IP, IF, FC, ELISA SMAD5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMAD5 fusion protein Ag2809
• Vollständiger Name: SMAD family member 5
• Berechnetes Molekulargewicht: 465 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC009682
• Gene symbol: SMAD5
• Gene ID (NCBI): 4090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.

Publikationen

Species	Application	Title
		PLoS Biol; BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.; Authors - Christian Hiepen
human	FC	Sci Rep; IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.; Authors - Gina Dörpholz
rat	WB	Int J Biochem Cell Biol; The Role of SIRT6 in the Differentiation of Vascular Smooth Muscle Cells in Response to Cyclic Strain.; Authors - Qing-Ping Yao
mouse	WB	Sci Rep; Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.; Authors - Xueni Wang
mouse	WB	PLoS One; Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.; Authors - Zhang Xing-Yi XY
human	WB	Biochem Biophys Res Commun; SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.; Authors - Jinliang Chen