SMAD6 Polyklonaler Antikörper

SMAD6 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18342-1-AP

Synonyme

hSMAD6, MADH6, Mothers against DPP homolog 6, SMAD family member 6

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  WB analysis using 18342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18342-1-AP (SMAD6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18342-1-AP (SMAD6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18342-1-AP bindet in WB, ELISA SMAD6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMAD6 fusion protein Ag13128
• Vollständiger Name: SMAD family member 6
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC012986
• Gene symbol: SMAD6
• Gene ID (NCBI): 4091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD6 is an intracellular signal transduction protein that belongs to the SMAD protein family. It acts as a negative regulator in the transforming growth factor-beta (TGF-β) signaling pathway, binding to TGF-β receptors and inhibiting the phosphorylation of SMAD2/SMAD3, thereby blocking the transmission of TGF-β signals. Additionally, SMAD6 is involved in the regulation of the bone morphogenetic protein (BMP) signaling pathway, affecting cell proliferation, differentiation, and apoptosis. During development, SMAD6 is crucial for the formation of bones and cartilage. Its abnormal expression is associated with various diseases, including skeletal developmental abnormalities and certain types of cancer.

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.; Authors - Wei Lu