SMAD7 Monoklonaler Antikörper

SMAD7 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D1C3

Kat-Nr. : 68594-1-PBS

Synonyme

MADH7, MAD homolog 8, MAD homolog 7, hSMAD7, CRCS3

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  WB analysis using 68594-1-Ig (same clone as 68594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68594-1-Ig (SMAD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68594-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68594-1-Ig (same clone as 68594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMAD7 antibody (68594-1-Ig, Clone: 3D1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68594-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 68594-1-Ig (same clone as 68594-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMAD7 Monoclonal antibody (68594-1-Ig, Clone:3D1C3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68594-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68594-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA SMAD7 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMAD7 fusion protein Ag13688
• Vollständiger Name: SMAD family member 7
• Berechnetes Molekulargewicht: 426 aa, 46 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC074819
• Gene symbol: SMAD7
• Gene ID (NCBI): 4092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.